Effective Amplification of 20-kb DNA by Reverse Transcription PCR

One-Step Reverse Transcription PCR (RT-PCR). Fatemeh Mehrpooyan*

Identification of introns by reverse-transcription PCR.

analyzing the gene of interest has several advantages over subcloning analysis. This method does not require any information about physical maps of the insert DNA. Positions of transposon insertion are presented as a distance from one end of the genomic DNA. Even if the restriction map is known, this technique should be preferable because of its simplicity. Using this protocol, it takes only on...

Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase.

A recombinant DNA polymerase derived from the thermophilic eubacterium Thermus thermophilus (Tth pol) was found to possess very efficient reverse transcriptase (RT) activity in the presence of MnCl2. Many of the problems typically associated with the high degree of secondary structure present in RNA are minimized by using a thermostable DNA polymerase for reverse transcription, and predominantl...

PCR Amplification of Reverse - transcribed mRNA

We reported that interleukin (IL) 6 alone cannot induce osteoclast formation in cocultures of mouse bone marrow and osteoblastic cells, but soluble IL-6 receptor (IL-6R) strikingly triggered osteoclast formation induced by IL-6. In this study, we examined the mechanism of osteoclast formation by IL-6 and related cytokines through the interaction between osteoblastic cells and osteoclast progeni...

Recombinant DNA sequences generated by PCR amplification.

The polymerase chain reaction (PCR) has greatly enhanced the field of molecular biology by making numerous regions of the genome (coding and noncoding), in both extant and extinct taxa, accessible for detailed analysis. PCR is especially well suited for applications in systematic biology because conserved regions that flank variable portions of the genome can be used as primer sites for the amp...